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The effect of different oxygen concentrations on stemness of hematopoietic stem cells
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F. Mohammadali    , M. Jamali |
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Abstract: (291 Views) |
Abstract
Background and Objectives
Recently, studies on the factors involved in stemness of stem cells have received a lot of attention due to its importance of stem cell-based treatments. One of the important stimuli in the fate of stem cells is the amount of oxygen in the environment. The results of various studies showed that a low concentration of oxygen in the niche of stem cells, preserves the reserves of the stem cells. Therefore, this study aims to investigate the effect of hypoxia on the hematopoietic stem cells (HSC) and the involved mechanisms.
Materials and Methods
In this review article, more than 100 papers in the pubmed database were reviewed. In this study, hypoxia induction methods, the effect of hypoxia on HSC and the effect of hypoxia on HSCs in Co-culture with other cells, hypoxia relationship with HIF1a factor and hypoxia relationship with stem cell stemness were discussed.
Results
The results of this review showed that low oxygen concentration can affect the stemness of stem cells. The difference in the results observed in different studies was due to different hypoxia induction methods, oxygen percentage, type of stem cells and time exposed to hypoxia, which necessitated optimization of the protocols for hypoxia induction.
Conclusions
Compared to the oxygen concentration of the environment, very low concentrations of oxygen (anoxia: 1%) take the stem cells mainly in the dormant phase and maintain a high stemness state, while at higher concentrations (5%) along with maintaining the proliferative potential of cells, stemness is also maintained. Of course, designing optimal culture conditions with specific oxygen concentration and understanding the mechanisms involved can help in the development of new target molecules and treatments based on stem cell in various diseases.
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| Keywords: Key words: Hypoxia, Oxygen, Hematopoietic Stem Cells
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Full-Text [DOCX 331 kb]
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Type of Study: Review Article |
Subject:
Stem cells
Published: 2024/03/29
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