|
Investigating natural mutations in BCP and preC regions of hepatitis B virus in blood donors with chronic hepatitis B anti-HBe+
|
Z. Sharifi    , A. Yadegari , Z. Paz |
|
|
| Keywords: Key words: Mutation, Hepatitis B Virus, Blood donors |
|
|
Full-Text [PDF 490 kb]
(63 Downloads)
| Abstract (HTML) (174 Views)
|
Type of Study: Research |
Subject:
Virology
Published: 2024/03/29
|
|
|
|
|
|
|
Full-Text: (38 Views) |
References:
- Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: G-LOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin ; 68(6): 394-424.
- Kramvis A. Genotypes and genetic variability of hepatitis B virus. Intervirology 2014; 57(3-4): 141-50.
- Alexopoulou A, Karayiannis P. HBeAg negative variants and their role in the natural history of chronic hepatitis B virus infection. World J Gastroenterol 2014; 20(24): 7644-52.
- Ortega-Prieto AM, Dorner M. Immune Evasion Strategies during Chronic Hepatitis B and C Virus Infection. Vaccines (Basel) 2017; 5(3): 24.
- Tu T, Budzinska MA, Shackel NA, Urban S. HBV DNA Integration: Molecular Mechanisms and Clinical Implications. Viruses 2017; 9(4): 75.
- Bahrami A, Sharifi Z, Parsania M, Haghighat S. Prevalence of anti-HBc in HBsAg negative blood donors using two enzyme immunoassays kits. Sci J Iran Blood Transfus Organ 2020; 17(2): 83-90. [Article in Farsi]
- Nowak MA, Bonhoeffer S, Hill AM, Boehme R, Thomas HC, McDade H. Viral dynamics in hepatitis B virus infection. Proc Natl Acad Sci U S A 1996; 93: 4398–4402.
- Okamoto H, Imai M, Kametani M, Nakamura T, Mayumi M. Genomic heterogeneity of hepatitis B virus in a 54-yearold woman who contracted the infection through maternofetal transmission. Jpn J Exp Med 1987; 57: 231-6.
- Yang ZT, Huang SY, Chen L, Liu F, Cai XH, Guo YF, et al. Characterization of Full-Length Genomes of Hepatitis B Virus Quasispecies in Sera of Patients at Different Phases of Infection. J Clin Microbiol 2015; 53(7): 2203-14.
- Zhang D, Ma S, Zhang X, Zhao H, Ding H, Zeng C. Prevalent HBV point mutations and mutation combinations at BCP/preC region and their association with liver disease progression BMC Infect Dis 2010; 10: 271.
- Kim H, Lee SA, Do SY, Kim BJ. Precore/core region mutations of hepatitis B virus related to clinical severity. World J Gastroenterol 2016; 22(17): 4287-96.
- Okamoto H, Tsuda F, Akahane Y, Sugai Y, Yoshiba M, Moriyama K, et al. Hepatitis B virus with the mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen. J Virol 1994; 68: 8102-10.
- Ghabeshi S, Sharifi Z, Hosseini SM, Shooshtari MM. Correlation between viral load of HBV in chronic hepatitis B patients and precore and Basal core promoter mutations. Hepat Mon 2013; 13(2): e7415.
- Chachá SGF, Gomes-Gouvêa MS, Malta FdM, Ferreira SdC, Villanova MG, Souza FF, et al. Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases. Mem Inst Oswaldo Cruz 2017; 112(9): 626-31.
- Ouneissa R, Bahri O, Alaya-Bouafif NB, Chouaieb S, Ben Yahia A, Sadraoui A, et al. Frequency and clinical significance of core promoter and precore region mutations in Tunisian patients infected chronically with hepatitis B. J Med Virol 2012; 84(11): 1719-26.
- Sanaei N, Hashemi SMA, Dehno SZS, Asl MM, Moini M, Malek-Hosseini SA, Hosseini SY, Sarvari J. Precore/core mutations of hepatitis B virus genotype D arising in different states of infection. Clin Exp Hepatol 2022; 8(1): 21-8.
- Kramvis A, Kew MC. Relationship of genotypes of hepatitis B virus to mutations, disease progression and response to antiviral therapy. J Viral Hepat 2005; 12: 456-64.
- Rodriguez-Frias F, Buti M, Jardi R, Cotrina M, Viladomiu L, Esteban R, Guardia J. Hepatitis B virus infection: precore mutants and its relation to viral genotypes and core mutations. Hepatology 1995; 22: 1641-7.
- Feng C, Cao LJ, Song HF, Xu P, Chen H, Xu JC, et al. Expression of PD-L1 on CD4+CD25+Foxp3+ Regulatory T Cells of Patients with Chronic HBV Infection and Its Correlation with Clinical Parameters. Viral Immunol 2015; 28(8): 418-24.
- Bertoletti A, Ferrari C. Adaptive immunity in HBV infection. J Hepatol 2016; 64(1 Suppl): S71-S83.
- Boni C, Laccabue D, Lampertico P, Giuberti T, Viganò M, Schivazappa S, et al . Restored function of HBV-specific T cells after long-term effective therapy with nucleos(t)ide analogues. Gastroenterology 2012; 143(4): 963-73.e9.
- Carman WF, Thomas HC, Domingo E. Genetic variation in viruses: clinical relevance to HBV. Lancet 1993; 341: 349-53.
|
Sci J Iran Blood Transfus Organ 2024;21 (1): 1-8
|
Investigating natural mutations in BCP and preC regions
of hepatitis B virus in blood donors with chronic hepatitis
B anti-HBe+
Sharifi Z.1, Yadegari A.1, Paz Z.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Various mutations can occur in the HBV genome during chronic HBV infection due to error-prone viral replication and stress on the host immune system. This study aimed to determine the mutations in the BCP and preC regions of the hepatitis B virus genome in asymptomatic carriers and its role in the persistence of hepatitis B infection.
Materials and Methods
In this descriptive study conducted by easy non-probability sampling method in 2017, 40 asymptomatic blood donors positive in terms of HBsAg and confirmatory tests and negative in terms of anti-HCV and anti-HIV tests were included in the study. Anti-HBc, HBe Ag and anti-HBe (Total) tests and ALT and AST tests were performed. Nested-PCR reaction was performed on the extracted DNA and the sequences were determined. Data analysis was done using online software https://hbv.geno2pheno.org.
Results
Out of the 40 examined samples (94.8%), 38 were men and 2 (5.2%) women with an average age of 38.6 ± 8.6. Mutations in (G1764A) and BCP (A1762T) were observed in 10 (50%) and 8 (40%) cases, respectively, and G54T17 mutation was observed in 3 (15%) samples out of 20 HBV positive and HBeAg negative samples. The mutation in the preC region and the nucleotide position A1896 G was found in 12 cases (60%).
Conclusions
The highest frequency of mutations was observed in G1764A and BCP which can be considered as one of the effective factors in not clearing and removing the virus by the host's immune system and causing chronic hepatitis B infection.
Key words: Mutation, Hepatitis B Virus, Blood donors
Received: 11 Nov 2023
Accepted: 28 Nov 2023
Correspondence: Sharifi Z., PhD of Virology. Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052152; Fax: (+9821) 88601555
E-mail: sharifiz@yahoo.com
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
|
Sharifi Z, Yadegari A, Paz Z. Investigating natural mutations in BCP and preC regions of hepatitis B virus in blood donors with chronic hepatitis B anti-HBe+. Sci J Iran Blood Transfus Organ 2024; 21 (1) :1-8 URL: http://bloodjournal.ir/article-1-1515-en.html
|
.jpg)
.png)
